反应详情

温度

0 °C

Methanesulfonyl chloride (0.55 mL) was added to a solution of 3-[2-(3-{[tert-butyl(dimethyl)silyl]oxy}-2,2-dimethylpropyl)-4-(hydroxymethyl)-1-oxo-1,2-dihydroisoquinolin-6-yl]-N-cyclopropyl-5-fluoro-4-methylbenzamide (Example 26 g, 3.65 g) and triethylamine (1.79 mL) in DCM (10 mL) at −20° C. After 30 min the solution was warmed to 0° C. and tert-butyl (3R)-3-methylpiperazine-1-carboxylate (2.58 g) was added. The reaction was stirred for 18 h at room temperature, evaporated and the residue purified (SiO2 chromatography, elution with 20 to 80% ethyl acetate in isohexane). Fractions containing product were evaporated, dissolved in THF (100 mL) and treated with 4 M HCl in dioxane (5 mL), stirred at room temperature for 60 h, evaporated to dryness and the residue purified (SiO2 chromatography, elution with 5 to 10% 7N NH3/methanol in DCM) to give the title compound (1.53 g) as a solid.

1. customevaporated
2. customthe residue purified
3. wash(SiO2 chromatography, elution with 20 to 80% ethyl acetate in isohexane)
4. additionFractions containing product
5. customwere evaporated
6. dissolutiondissolved in THF (100 mL)
7. additiontreated with 4 M HCl in dioxane (5 mL)
8. stirringstirred at room temperature for 60 h
9. customevaporated to dryness
10. customthe residue purified
11. wash(SiO2 chromatography, elution with 5 to 10% 7N NH3/methanol in DCM)