反应详情

To a solution of 2-{[4-(2-{[(9H-fluorene-9-ylmethoxy)carbonyl]amino}ethyl)-1,3-thiazol-2-yl]thio}-2-methylpropionic acid tert-butyl ester (33 g) synthesized in Example 483-1 in dichloromethane (30 mL) was added trifluoroacetic acid (50 mL), and the mixture was stirred at room temperature overnight. The reaction mixture was poured into water, extracted with ethyl acetate. The organic layer was washed with water and saturated brine, and dried over sodium sulfate. The solvent was concentrated under reduced pressure, and purified by silica gel chromatography (elution solvent; ethyl acetate) to give the title compound (38 g) as a pale-brown oil.

1. extractionextracted with ethyl acetate
2. washThe organic layer was washed with water and saturated brine
3. dry with materialdried over sodium sulfate
4. concentrationThe solvent was concentrated under reduced pressure
5. custompurified by silica gel chromatography (elution solvent; ethyl acetate)