Sodium Chloride → Atrolactic acid

HRID 917264Notes

Reaction equation

HRID 917264 reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11]O)O)O)O)[OH:4].[Na+].[Cl-].Cl.N[C@H:17](C(O)=O)[CH2:18]S.[C:23](=[O:26])([O-])[O-:24].[Ca+2]>>[C:5]1([C:3]([CH3:2])([OH:4])[C:23]([OH:24])=[O:26])[CH:18]=[CH:17][CH:11]=[CH:9][CH:7]=1

Participants

5

Reactant 4

HCID 5234Sodium ChlorideHCID 10112Calcium CarbonateHCID 60960Cysteine HydrochlorideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 1303Atrolactic acid

Conditions

Temperature
30 °C

Procedure

210 parts by weight of this glucose was mixed with 11,600 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 116 parts by weight of a pre-culture fluid of phenyllactic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 3 days. Thereafter, the procedure in Example 1 was repeated to collect 9 parts by weight of phenyllactic acid.

Workup

  1. customthe mixture formed
  2. additionwas then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes)
  3. additionThereafter, the culture medium treated

Sources & evidence

ORD record
ord-cd832ad6fa7a48da9f9f3abe335c672a
Source dataset
uspto-grants-2007_09
Patent number
US07273734B2

Reaction notes

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