Sodium Chloride → HCID 86587261, compound
Reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])O)O)O.[Na+].[Cl-].Cl.N[C@H:17]([C:20]([OH:22])=[O:21])[CH2:18]S.C(=O)([O-])[O-:24].[Ca+2]>>[OH:24][CH:17]([CH2:18][CH:3]([CH3:5])[CH3:2])[C:20]([OH:22])=[O:21].[C:11]([OH:12])(=[O:21])[CH:9]([CH3:7])[OH:10]Participants
Conditions
- Temperature
- 30 °C
Procedure
200 parts by weight of this glucose was mixed with 11,000 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 110 parts by weight of a pre-culture fluid of 2-hydroxyisocaproic acid/lactic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 3 days. Thereafter, the culture fluid was filtered, and the water and volatile components of the filtrate obtained were evaporated off under normal-temperature reduced pressure, followed by NMR analysis. As a result, it was ascertained that a mixture of 10 parts by weight of 2-hydroxyisocaproic acid and 12 parts by weight of lactic acid was obtained.
Workup
- customthe mixture formed
- additionwas then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes)
- additionThereafter, the culture medium treated
Sources & evidence
- ORD record
- ord-d37b30455a2949818347f1a63d9d65c0
- Source dataset
- uspto-grants-2007_09
- Patent number
- US07273734B2
Reaction notes
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