Sodium Chloride → Atrolactic acid

HRID 917277Notes

Reaction equation

HRID 917277 reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([CH2:9]O)O)O)[OH:4].[Na+].[Cl-].Cl.N[C@H:15]([C:18](O)=O)[CH2:16]S.[C:21](=[O:24])([O-])[O-:22].[Ca+2]>>[C:5]1([C:3]([CH3:2])([OH:4])[C:21]([OH:22])=[O:24])[CH:18]=[CH:15][CH:16]=[CH:9][CH:7]=1

Participants

5

Reactant 4

HCID 5234Sodium ChlorideHCID 10112Calcium CarbonateHCID 60960Cysteine HydrochlorideHCID 644160aldehydo-D-Xylose

Product 1

HCID 1303Atrolactic acid

Conditions

Temperature
30 °C

Procedure

550 parts by weight of this xylose was mixed with 27,000 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 270 parts by weight of a pre-culture fluid of phenyllactic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 6 days. Thereafter, the culture fluid was filtered, and the water and volatile components of the filtrate obtained were evaporated off under normal-temperature reduced pressure, followed by purification to obtain 10 parts by weight of phenyllactic acid.

Workup

  1. customthe mixture formed
  2. additionwas then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes)
  3. additionThereafter, the culture medium treated

Sources & evidence

ORD record
ord-571599f79e924103b4510ce14c07e773
Source dataset
uspto-grants-2007_09
Patent number
US07273734B2

Reaction notes

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