Sodium Chloride → (+-)-2-Hydroxyisocaproic acid

HRID 917278Notes

Reaction equation

HRID 917278 reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H](CO)O)O)O.[Na+].[Cl-].Cl.N[C@H:15]([C:18]([OH:20])=[O:19])[CH2:16]S.C(=O)([O-])[O-:22].[Ca+2]>>[OH:22][CH:15]([CH2:16][CH:3]([CH3:5])[CH3:2])[C:18]([OH:20])=[O:19]

Participants

5

Reactant 4

HCID 5234Sodium ChlorideHCID 10112Calcium CarbonateHCID 60960Cysteine HydrochlorideHCID 644160aldehydo-D-Xylose

Product 1

HCID 92779(+-)-2-Hydroxyisocaproic acid

Conditions

Temperature
30 °C

Procedure

550 parts by weight of this xylose was mixed with 27,000 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 270 parts by weight of a pre-culture fluid of 2-hydroxyisocaproic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 6 days. Thereafter, the culture fluid was filtered, and the water and volatile components of the filtrate obtained were evaporated off under normal-temperature reduced pressure, followed by purification to obtain 12 parts by weight of 2-hydroxyisocaproic acid.

Workup

  1. customthe mixture formed
  2. additionwas then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes)
  3. additionThereafter, the culture medium treated

Sources & evidence

ORD record
ord-a4e4c5ef65644152a771bfa06ebe8fb2
Source dataset
uspto-grants-2007_09
Patent number
US07273734B2

Reaction notes

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