反应详情

温度

65 °C

Palmitic acid methyl ester (2.00 g; 7.40 mmol) was combined with poly(ethylene glycol) (PEG-12) monomethyl ether 550 (4.41 g; 7.40 mmol; 1.0 equiv) having an average molecular weight of 596 (available from Sigma-Aldrich Chemical Company) in a 40-milliliter vial equipped with a magnetic stir bar. Immobilized Candida antarctica lipase B immobilized on a porous fluoropolymer support as described in US published Patent Application 2012/0040395 was added to the flask in an amount of 0.13 grams, (2 wt % based on the total weight of starting materials). The mixture was stirred and heated to 65° C. and purged with a subsurface stream of nitrogen at a rate of 100 mL/min. After 24 hours a final sample was removed and analyzed by GC to determine conversion of methyl palmitate to palmitic acid methyl ester ethoxylate. Conversion was as follows: after 3 hours, 64.8%; after 6 hours, 80.8%, after 12 hours, 92.1%; and after 23 hours, 96.4%.

1. customvial equipped with a magnetic stir bar
2. additionwas added to the flask in an amount of 0.13 grams
3. custompurged with a subsurface stream of nitrogen at a rate of 100 mL/min
4. customAfter 24 hours a final sample was removed
5. waitafter 6 hours
6. wait80.8%, after 12 hours
7. waitand after 23 hours