Trifluoroacetic acid → 1-(4-Methoxy-benzenesulfonyl)-1H-pyrrole-3-carboxylic acid (6-amino-2,4-dimethyl-pyridin-3-ylmethyl)-amide

HRID 928384Notes

Reaction equation

HRID 928384 reaction equation
C(OC(=O)[NH:7][C:8]1[CH:13]=[C:12]([CH3:14])[C:11]([CH2:15][NH:16][C:17]([C:19]2[CH:23]=[CH:22][NH:21][CH:20]=2)=[O:18])=[C:10]([CH3:24])[N:9]=1)(C)(C)C.CC([O-])(C)C.[K+].[CH3:32][O:33][C:34]1[CH:39]=[CH:38][C:37]([S:40](Cl)(=[O:42])=[O:41])=[CH:36][CH:35]=1.C(O)(C(F)(F)F)=O>CN(C1C=CN=CC=1)C.C1COCC1.CO.O.C(Cl)Cl>[NH2:7][C:8]1[N:9]=[C:10]([CH3:24])[C:11]([CH2:15][NH:16][C:17]([C:19]2[CH:23]=[CH:22][N:21]([S:40]([C:37]3[CH:36]=[CH:35][C:34]([O:33][CH3:32])=[CH:39][CH:38]=3)(=[O:42])=[O:41])[CH:20]=2)=[O:18])=[C:12]([CH3:14])[CH:13]=1

Participants

10

Reactant 4

HCID 6422Trifluoroacetic acidHCID 74014-Methoxybenzenesulfonyl chlorideHCID 23665647Potassium tert-butoxideHCID 58081282tert-butyl 5-((1H-pyrrole-3-carboxamido)methyl)-4,6-dimethylpyridin-2-ylcarbamate

Product 1

HCID 580811581-(4-Methoxy-benzenesulfonyl)-1H-pyrrole-3-carboxylic acid (6-amino-2,4-dimethyl-pyridin-3-ylmethyl)-amide

Reagents, catalysts & solvents 5

HCID 142844-(Dimethylamino)pyridineCatalystHCID 887MethanolSolventHCID 962WaterSolventHCID 6344DichloromethaneSolventHCID 8028TetrahydrofuranSolvent2×

Procedure

To a mixture of (4,6-dimethyl-5-{[(1H-pyrrole-3-carbonyl)-amino]-methyl}-pyridin-2-yl)-carbamic acid tert-butyl ester (45 mg, 0.131 mmol) and 4-DMAP (6.38 mg, 0.052 mmol) in dry THF (0.8 ml) was added under argon atmosphere a 1.0 M solution of KOtBu in THF (0.196 ml, 0.196 mmol). The reaction mixture was stirred at it for 20 min. 4-Methoxy-benzenesulfonyl chloride (32.4 mg, 0.157 mmol) was added in one portion and the mixture was stirred for an additional 17 h at rt. The reaction mixture was diluted with MeOH (1 ml) and H2O (1 ml) and the resulting solution was filtered over a PTFE membrane (0.45 um). The filtrate was purified by a preparative LC-MS system using a Waters Sunfire™ C-18 OBD column [150×30 mm, 5 um particle size] and elution at 50 ml/min using the following gradient: 0-1.5 min (90% water containing 0.1% TFA/10% acetonitrile), 1.5-11.5 min (linear gradient from 90% water containing 0.1% TFA/10 acetonitrile to 35% water containing 0.1% TFA/65% acetonitrile), 11.5-12.5 min (linear gradient from 35% water containing 0.1% TFA/65% acetonitrile to 0% water containing 0.1% TFA/100% acetonitrile), 12.5-13.5 min (0% water containing 0.1% TFA/100% acetonitrile). Product collection was triggered by the MS signal and the resulting fractions were pooled and freeze-dried to yield a colorless powder. A 50% solution of TFA in DCM (1.0 ml) was added and the reaction mixture was stirred at rt for 2 h. After evaporation to dryness, the residue was dissolved in a mixture of acetonitrile/water and freeze-dried to yield the title compound. 1H-NMR (DMSO, 400 MHz): 13.52 (br s, 1H), 8.26 (t, 1H), 7.96-7.92 (m, 2H), 7.87-7.86 (m, 1H), 7.68 (br s, 2H), 7.37 (t, 1H), 7.19-7.16 (m, 2H), 6.67-6.66 (m, 1H), 6.63 (s, 1H), 4.26 (d, 2H), 3.85 (s, 3H), 2.50 (s, 3H), 2.37 (s, 3H); LCMS (Method T) RtT=0.88 min; [M+H]+=414.8.

Workup

  1. stirringthe mixture was stirred for an additional 17 h at rt
  2. filtrationthe resulting solution was filtered over a PTFE membrane (0.45 um)
  3. customThe filtrate was purified by a preparative LC-MS system
  4. washa Waters Sunfire™ C-18 OBD column [150×30 mm, 5 um particle size] and elution at 50 ml/min
  5. custom0-1.5 min (90% water containing 0.1% TFA/10% acetonitrile), 1.5-11.5 min (linear gradient from 90% water containing 0.1% TFA/10 acetonitrile to 35% water containing 0.1% TFA/65% acetonitrile), 11.5-12.5 min (linear gradient from 35% water containing 0.1% TFA/65% acetonitrile to 0% water containing 0.1% TFA/100% acetonitrile), 12.5-13.5 min (0% water containing 0.1% TFA/100% acetonitrile)
  6. customProduct collection
  7. customfreeze-dried
  8. customto yield a colorless powder
  9. stirringthe reaction mixture was stirred at rt for 2 h
  10. customAfter evaporation to dryness
  11. dissolutionthe residue was dissolved in a mixture of acetonitrile/water
  12. customfreeze-dried

Sources & evidence

ORD record
ord-c305f8f92e2c4a35aeeec584c70d5b42
Source dataset
uspto-grants-2016_03
Patent number
US09290485B2

Reaction notes

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