反应详情

温度

100 °C

(3S,5R,6S)-3-allyl-5-(3-chlorophenyl)-6-(5-chloropyridin-2-yl)-1-((S)-1-hydroxybutan-2-yl)-3-methylpiperidin-2-one (Example 121, Step L, 100 mg, 0.224 mmol) was azeotroped three times in benzene on a rotary evaporator. The residue was dissolved in toluene and transferred to a reaction vessel. The vessel was flushed with argon. Ethanethiol (33.1 μl, 0.447 mmol) was added followed by cyanomethylenetributylphosphorane (216 μl, 0.894 mmol). The vessel was sealed and the solution was heated to 100° C. for 4 h. The reaction mixture was diluted with DCM (10 ml) and Si-maleimide (Silicycle, 2.1 g; 0.66 mmol/g; 40-63 microns) was added to scavenge excess thiol. After stirring for about 1 h, the mixture was filtered and the silica gel was rinsed with DCM. The filtrate was concentrated on a rotary evaporator. The residue was dissolved in DCM and loaded directly onto a dry 12 g gold-capped Redisep® column (Teledyne Isco, Lincoln, Nebr.). The column was eluted with a gradient of 0 to 5% MeOH:DCM. The fractions containing the desired product were combined and concentrated to afford the title compound as a colorless film.

1. customon a rotary evaporator
2. dissolutionThe residue was dissolved in toluene
3. customtransferred to a reaction vessel
4. customThe vessel was flushed with argon
5. customThe vessel was sealed
6. additionwas added
7. filtrationthe mixture was filtered
8. washthe silica gel was rinsed with DCM
9. concentrationThe filtrate was concentrated on a rotary evaporator
10. dissolutionThe residue was dissolved in DCM
11. customcapped Redisep® column (Teledyne Isco, Lincoln, Nebr.)
12. washThe column was eluted with a gradient of 0 to 5% MeOH
13. additionThe fractions containing the desired product
14. concentrationconcentrated