Potassium Chloride → Phosphate Buffered Saline

HRID 962855Notes

Reaction equation

HRID 962855 reaction equation
[Cl-:1].[Na+:2].[Cl-].[K+:4].[P:5]([O-:9])([O-:8])([OH:7])=[O:6].[Na+].[Na+].[P:12]([O-:16])([OH:15])([OH:14])=[O:13].[K+].[N-]=[N+]=[N-].[Na+]>O>[OH:7][P:5]([O-:9])([OH:8])=[O:6].[OH:14][P:12]([O-:16])([O-:15])=[O:13].[Na+:2].[Na+:2].[Na+:2].[Cl-:1].[Cl-:1].[K+:4].[K+:4]

Participants

7

Reactant 5

HCID 4873Potassium ChlorideHCID 5234Sodium ChlorideHCID 24203Sodium Phosphate, DibasicHCID 33557Sodium AzideHCID 516951Monobasic potassium phosphate

Product 1

HCID 24978514Phosphate Buffered Saline

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

Dulbecco's phosphate buffered saline (DPBS) is prepared by first dissolving, sequentially, about 160 g sodium chloride (NaCI), about 4 grams potassium chloride (KCl), about 23 grams disodium hydrogen orthophosphate (Na2HPO4), about 4 grams potassium dihydrogen orthophosphate (KH2PO4), and about 10 grams sodium azide in a liter of reverse-osmosis (MilliQ) water. Then, the pH is adjusted to about 7.3 by adding appropriate amounts of HCI. Finally, the buffer solution is diluted to 1:20 (50 ml buffer solution with 950 ml reverse-osmosis water). and allowed to degas either in a screw-capped container overnight or under vacuum.

Workup

  1. additionby adding appropriate amounts of HCI
  2. customto degas either in a screw-capped container overnight or under vacuum

Sources & evidence

ORD record
ord-011088ed1ff140be94004e58e4f74295
Source dataset
uspto-grants-1998_06
Patent number
US05760100

Reaction notes

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