Potassium Chloride → Phosphate Buffered Saline

Participants

Reaction Steps

Dulbecco's phosphate buffered saline (DPBS) is prepared by first dissolving, sequentially, about 160 g sodium chloride (NaCI), about 4 grams potassium chloride (KCl), about 23 grams disodium hydrogen orthophosphate (Na2HPO4), about 4 grams potassium dihydrogen orthophosphate (KH2PO4), and about 10 grams sodium azide in a liter of reverse-osmosis (MilliQ) water. Then, the pH is adjusted to about 7.3 by adding appropriate amounts of HCI. Finally, the buffer solution is diluted to 1:20 (50 ml buffer solution with 950 ml reverse-osmosis water). and allowed to degas either in a screw-capped container overnight or under vacuum.

Provenance & Evidence