反应详情

温度

25 °C

To a suspension of 912 mg of 2-methyl-4-(6-carbomethoxyhexyl)-5-(3-keto-1-trans-octenyl)-thiazole (Ia, R1 = CH3) in 20 ml of 0.2 M phosphate buffer of pH 7.5, 90 mg of the lipase enzyme specified in Example 5, 9 mg of the sodium salt of taurocholic acid nd 0.9 ml of a 10 % solution of gum arabic were added. The mixture was then shaken at 25° C in a screening shaker desk at 260 revolutions/minute and an amplitude of 2 cm for 24 hours. Then the reaction mixture was diluted with 50 ml of water, acidified with citric acid and extracted with 3 × 40 ml of ethyl acetate. The ethyl acetate extract was dried over sodium sulphate and evaporated in vacuo. The obtained 1.03 g of evaporation residue were purified by preparative thin layer chromatography in the way specified in Example 6. Thus, 645 mg of 2-methyl-4-(6-carboxyhexyl)-5-(3-keto-1-trans-octenyl)-thiazole Ib, R1 = CH3) were obtained.

1. additionwere added
2. extractionextracted with 3 × 40 ml of ethyl acetate
3. extractionThe ethyl acetate extract
4. dry with materialwas dried over sodium sulphate
5. customevaporated in vacuo
6. customThe obtained 1.03 g of evaporation residue
7. customwere purified by preparative thin layer chromatography in the way