反应详情

To 75 ml of an ethyl acetate solution of Nα -benzyloxycarbonyl-L-histidine azide (4) prepared from 4.85 g of Nα -benzyloxycarbonyl-L-histidine hydrazide (3) by the method of Reference example 2 was added 2.23 g of compound (31) under ice-cooling and the mixture was placed overnight in a refrigerator to maintain the reaction. The reaction mixture was concentrated and the residue was subjected to silica gel column chromatography. By eluting the product with chloroform-methanol-aqueous ammonia (95:5:0.5), 4.13 g of Nα -benzyloxycarbonyl-L-histidyl-N-butyl-L-prolinamide was obtained.

1. temperaturecooling
2. temperatureto maintain
3. customthe reaction
4. concentrationThe reaction mixture was concentrated
5. washBy eluting the product with chloroform-methanol-aqueous ammonia (95:5:0.5)