反应详情

Maltotetraose is the preferred α(1→4) linked glucan for use in the present invention. Maltotetraose is an oligosaccharide consisting of four α(1→4) linked glucose units and is commercially available from Sigma Chemical Company, St. Louis, Missouri, or Boehringer Mannheim, GmbH, Mannheim, West Germany. Where maltotetraose is employed as the α(1→4) linked glucan, the kinetic assay proceeds as follows: Maltotetraose substrate is hydrolyzed by α-amylase in the test sample to yield two moles of maltose per mole of substrate. The enzyme maltose phosphorylase (MP) catalyzes the phosphorolysis of each mole of maltose to one mole each of glucose and β-D-glucose-1-phosphate. The enzyme β-phosphoglucomutase (β-PGM) converts the β-glucose-1-phosphate to glucose-6-phosphate which is then oxidized to 6-phosphogluconate with the concomitant reduction of nicotinamide adenine dinucleotide (NAD) to NADH in the reaction catalyzed by G-6-PDH.