Thymidine → Thymidine, 5'-azido-5'-deoxy-
Reaction equation
[C@@H:1]1([N:9]2[CH:17]=[C:15]([CH3:16])[C:13](=[O:14])[NH:12][C:10]2=[O:11])[O:8][C@H:5]([CH2:6]O)[C@@H:3]([OH:4])[CH2:2]1.[N-:18]=[N+:19]=[N-:20].[Li+].C1(P(C2C=CC=CC=2)C2C=CC=CC=2)C=CC=CC=1.C(Br)(Br)(Br)Br>>[N:18]([CH2:6][C@H:5]1[O:8][C@@H:1]([N:9]2[CH:17]=[C:15]([CH3:16])[C:13](=[O:14])[NH:12][C:10]2=[O:11])[CH2:2][C@@H:3]1[OH:4])=[N+:19]=[N-:20]Participants
Procedure
Thymidine (2.42 g, 10.0 mmol) was dried under reduced pressure for 1 hour. Thereto was added 2.20 g (4.5 eq) of lithium azide, and the mixture was further dried for 1 hour. Distilled DMF (50 ml) was added to the reaction vessel, and the pressure was reduced for about 10 minutes with stirring. Further, 3.41 g (1.3 eq) of triphenylphosphine was added and, after confirmation of the dissolution of triphenylphosphine, 4.97 g (1.5 eq) of carbon tetrabromide was added to initiate the reaction. On that occasion, the solution turned yellow. After 40 minutes, the completion of the reaction was confirmed by TLC, and the reaction was quenched with methanol. The solution was distilled under reduced pressure, and the residue was purified by silica gel column chromatography (mobile phase MeOH:CHCl3=1:9) to give 5′-azido-5′-deoxythymidine.
Workup
- customthe mixture was further dried for 1 hour
- additionDistilled DMF (50 ml) was added to the reaction vessel
- additionwas added
- customthe reaction
- waitAfter 40 minutes
- customthe reaction was quenched with methanol
- distillationThe solution was distilled under reduced pressure
- customthe residue was purified by silica gel column chromatography (mobile phase MeOH:CHCl3=1:9)
Sources & evidence
- ORD record
- ord-ec3ed4f044a14a91a6275c0848e943a5
- Source dataset
- uspto-grants-2005_03
- Patent number
- US06872809B2
Reaction notes
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