反应详情

CC Acylase immobilized on HPA 25 (20 mL) and prepared in the above-mentioned (2) was packed in a column with a jacket, and 6.0 mg/mL glutaryl 7-aminocephalosporanic acid (GL7-ACA) solution (100 mL) was circulated for one hour at 20° C. at circulation flow rate of about 70 mL/min while adjusting the pH to 7.75 with NaOH, whereby 7-ACA was obtained. After the reaction, the reaction mixture in the column was pushed out with water and 7-ACA was recovered. The above-mentioned reaction was repeated 100 times (continuous batch reaction), which was taken as one cycle.