反应详情

Ralstonia eutropha NCIMB 11599 was cultured in a medium containing 10 g/L of glucose and 1 g/L of propionic acid, according to the method previously reported (Kim et al., Enzyme Microbial. Technol., 16: 556-561, 1994). Poly-(R)-(−)-3-hydroxybutyrate-co-(R)-(−)-3-hydroxyvalerate (PHB/V) copolymer (containing 8 mol % (R)-(−)-3-hydroxyvalerate) was accumulated to a content of 63% of cell dry weight, which was auto-degraded to produce (R)-(−)-3-hydroxybutyric acid and (R)-(−)-3-hydroxyvaleric acid. Cells were dispersed in water at a copolymer concentration of 27.4 g/L. Auto-degradation reaction was carried out at pH 7 and 30° C. for 35 hours. As a result, 5.8 g/L of (R)-(−)-3-hydroxybutyric acid and 0.6 g/L of (R)-(−)-3-hydroxyvaleric acid were obtained. Therefore, a mixture of (R)-(−)-3-hydroxybutyric acid and (R)-(−)-3-hydroxyvaleric acid can be efficiently prepared from the accumulated PHB/V polymer by auto-degradation of the copolymer, and they can be separated using LC or HPLC (Ref. Example 16).

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