Reaktionsgleichung
C1C=CC(N2C3(CCN(CCC[C:21]([C:23]4[CH:24]=[CH:25][C:26](F)=CC=4)=[O:22])CC3)C(=O)NC2)=CC=1.C(O)[C:31](N)([CH2:34][OH:35])[CH2:32]O.Cl.[Na+].[Cl-].[Mg+2].[Cl-].[Cl-].CC([C@]1(O)C[C@H]2[N:51](C[C@H]3C4C2=CC=CC=4CCC2C=CC=CC3=2)CC1)(C)C>>[NH2:51][CH2:26][CH2:25][C:24]1[CH:32]=[CH:31][C:34]([OH:35])=[C:21]([OH:22])[CH:23]=1Komponenten
6
Versuchsvorschrift
0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H] spiperone in a final volume of 1 mL containing 50 mM Tris HCl, 120 mM NaCl and 1 mM MgCl2 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol. After 15 minutes of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL).
Aufarbeitung
- custom0.5 mL aliquots of the membrane preparation
- filtrationsamples were filtered rapidly through Whatman GF/C glass filters under negative pressure
- washwashed three times with ice-cold binding buffer (5 mL)
Quellen und Nachweise
- ORD-Eintrag
- ord-343d3ac5b78c43dea6d4b98433875b34
- Quelldatensatz
- uspto-grants-1992_04
- Patentnummer
- US05109002
Verknüpfte Notizen
Wird geladen…