Phenylmethylsulfonyl Fluoride → L-Tyrosine

HRID 1067854

Reaktionsgleichung

Reaktionsgleichung von HRID 1067854
C(O)C(N)(CO)CO.CC(CC(C1C=[CH:21][C:20]([O:23]CCOCCO)=[CH:19][CH:18]=1)(C)C)(C)C.[F-].[Na+].C1(CS(F)(=O)=O)C=CC=CC=1.CC(C[C@H](NC(C)=O)C(N[C@H](C([NH:58][C@H:59]([C:67]([OH:69])=[O:68])[CH2:60][CH2:61][CH2:62]N=C(N)N)=O)CC(C)C)=O)C>OCC(CO)O>[NH2:58][C@H:59]([C:67]([OH:69])=[O:68])[CH2:60][C:61]1[CH:62]=[CH:21][C:20]([OH:23])=[CH:19][CH:18]=1

Komponenten

7

Edukt 5

HCID 4784Phenylmethylsulfonyl FluorideHCID 5235Sodium FluorideHCID 6503Tris(hydroxymethyl)aminomethaneHCID 24775Poly(oxy-1,2-ethanediyl), alpha-((1,1,3,3-tetramethylbutyl)phenyl)-omega-hydroxy-, potassium salt (1:1)HCID 189069Leupeptin acid

Produkt 1

HCID 6057L-Tyrosine

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 753GlycerolLösungsmittel

Versuchsvorschrift

Sf21 cells were infected with a baculovirus expression vector for BTK or SYK, as previously described (refs 18 and 19). Cells were harvested, lysed (10 mM Tris pH 7.6, 100 mM NaCl, 1% Nonidet P-40, 10% glycerol, 50 mM NaF, 100 mM Na3VO4, 50 μg/ml phenylmethylsulfonyl fluoride, 10 μg/ml aprotonin, 10 μg/ml leupeptin), and the kinase proteins were purified to homogeneity, as reported (refs 18 and 19).

Aufarbeitung

  1. customthe kinase proteins were purified to homogeneity

Quellen und Nachweise

ORD-Eintrag
ord-d995aca59e734259880615ad5c8cc936
Quelldatensatz
uspto-grants-2001_06
Patentnummer
US06248790B1

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