[C:1]([NH:5][C:6](=[O:23])[CH2:7][N:8]1[C:13](=[O:14])[C:12]2[C:15]([CH3:22])=[C:16]([C:18]([O:20]C)=[O:19])[S:17][C:11]=2[N:10]=[CH:9]1)([CH3:4])([CH3:3])[CH3:2].O.O.[OH-].[Li+]>C1COCC1.CO>[C:1]([NH:5][C:6](=[O:23])[CH2:7][N:8]1[C:13](=[O:14])[C:12]2[C:15]([CH3:22])=[C:16]([C:18]([OH:20])=[O:19])[S:17][C:11]=2[N:10]=[CH:9]1)([CH3:4])([CH3:3])[CH3:2]To a solution of methyl 3-(2-(tert-butylamino)-2-oxoethyl)-5-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylate, (3.3 g, 10 mmol) in THF (30 mL), MeOH (10 mL), and H2O (10 mL) was added lithium hydroxide monohydrate (1.26 g, 30 mmol). The reaction mixture was stirred at RT overnight. The reaction mixture was concentrated, diluted with H2O (30 mL) and washed with DCM (2×30 mL). The aqueous layer was acidified (pH˜2) with 1M HCl and the resultant precipitate was filtered and dried to give 1.8 g of 3-(2-(tert-butylamino)-2-oxoethyl)-5-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylic acid (yield: 55%).
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