5'-Adp → 5'-Atp

HRID 1274798

Reaktionsgleichung

Reaktionsgleichung von HRID 1274798
[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([OH:8])([OH:7])=[O:6])(=[O:3])[OH:2]>C(OP(O)(O)=O)[C@H]1O[C@H](OP(OP(O)(O)=O)(O)=O)[C@H](O)[C@@H]1O>[P:1]([O:9][CH2:10][C@H:11]1[O:15][C@@H:14]([N:16]2[C:25]3[N:24]=[CH:23][N:22]=[C:20]([NH2:21])[C:19]=3[N:18]=[CH:17]2)[C@H:13]([OH:26])[C@@H:12]1[OH:27])([O:4][P:5]([O:7][P:1]([OH:4])([OH:3])=[O:2])([OH:8])=[O:6])(=[O:2])[OH:3]

Komponenten

3

Edukt 1

HCID 60225'-Adp

Produkt 1

HCID 59575'-Atp

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 7339Phosphoribosyl PyrophosphateLösungsmittel3×

Versuchsvorschrift

The following additions were made to each reaction: 2 μl PRPP, 2 μl PRPP Synthetase, and 20 μl PRPP Synthetase buffer. The reactions proceeded at 37° C. for 28 min at which time the reactions were transferred to 100 μl LAR containing 2 μl ADP and 2 μl NDPK. This second reaction was allowed to proceed at room temperature for 20 min. The amount of ATP produced was measured by the addition of 10 ng of luciferase followed by measuring light output with a luminometer. The data is presented in Table 30.

Aufarbeitung

  1. customThe following additions were made to each reaction
  2. customThis second reaction
  3. waitto proceed at room temperature for 20 min

Quellen und Nachweise

ORD-Eintrag
ord-2891be8b47824b24942d2080ce26bd1f
Quelldatensatz
uspto-grants-2000_12
Patentnummer
US06159693

Verknüpfte Notizen

Wird geladen…