Penicillin G → 6-Aminopenicillanic acid

HRID 127997

Reaktionsgleichung

Reaktionsgleichung von HRID 127997
[CH3:1][C:2]1([CH3:23])[S:6][C@@H:5]2[C@H:7]([NH:10]C(CC3C=CC=CC=3)=O)[C:8](=[O:9])[N:4]2[C@H:3]1[C:20]([O-:22])=[O:21].[K+].CC1(C)S[C@@H]2[C@H](NC(CC3C=CC=CC=3)=O)C(=O)N2[C@H]1C(O)=O>P([O-])([O-])([O-])=O>[CH3:1][C:2]1([CH3:23])[S:6][C@@H:5]2[C@H:7]([NH2:10])[C:8](=[O:9])[N:4]2[C@H:3]1[C:20]([OH:22])=[O:21]

Komponenten

4

Edukt 2

HCID 5904Penicillin GHCID 23664709Penicillin G Potassium

Produkt 1

HCID 110826-Aminopenicillanic acid

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 1061PhosphateLösungsmittel

Versuchsvorschrift

The enzymatic activity of the penicillin amidase-support complex obtained is determined by contacting 0.5 g. of complex with 40 ml. of a 20 g./l. penicillin G solution in 0.1 M phosphate buffer, of pH 7.5, at 30° C., for 30 minutes. The complex is then removed by filtration and the 6-amino-penicillanic acid (6-APA) formed determined in the filtrate. The enzymatic activity of the complex is 50 U/g. (the unit "U" is the amount of complex which hydrolyzes 1 mircomol of penicillin G per minute).

Aufarbeitung

  1. customThe complex is then removed by filtration

Quellen und Nachweise

ORD-Eintrag
ord-59aca39157ce4510885ea6e0b42369e5
Quelldatensatz
uspto-grants-1981_03
Patentnummer
US04258133

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