Calcium Carbonate → L-(-)-Ornithine

HRID 1440303

Reaktionsgleichung

Reaktionsgleichung von HRID 1440303
C[C@H]1O[C@H]2O[C@H]3[C@H](O[C@@]2(O)C(=O)C1)[C@@H](NC)[C@@H](O)[C@@H](NC)[C@@H]3O.OC1O[C@H](CO)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O.OP([O-])(O)=O.[K+].OP([O-])([O-])=O.[K+].[K+].[NH2:60][C@H:61]([C:69]([OH:71])=[O:70])[CH2:62][CH2:63][CH2:64][NH:65]C(=N)N.C([O-])([O-])=O.[Ca+2]>>[NH2:60][C@H:61]([C:69]([OH:71])=[O:70])[CH2:62][CH2:63][CH2:64][NH2:65]

Komponenten

7

Edukt 6

HCID 10112Calcium CarbonateHCID 15541SpectinomycinHCID 24450Potassium Phosphate, DibasicHCID 440995(+)-LactoseHCID 516951Monobasic potassium phosphateHCID 178418600HCID 178418600, Verbindung

Produkt 1

HCID 6262L-(-)-Ornithine

Reaktionsbedingungen

Temperatur
30 °C

Versuchsvorschrift

Each strain was cultured with shaking in NB medium at 30° C. for 16 hours. For the culture of the transformant, spectinomycin was added to NB medium at a concentration of 100 μg/ml. Then, 0.5 ml of the seed culture was inoculated into 5 ml of a production medium (pH 7.0) comprising 80 g/l lactose, 40 g/l (NH4)2SO4, 0.5 g/l KH2PO4, 0.5 g/l K2HPO4, 100 mg/l L-arginine and 20 g/l CaCO3 in a test tube, and shaking culture was carried out at 30° C. for 72 hours. After the culturing was finished, the culture filtrate was subjected to paper chromatography and the amount of L-ornithine formed was determined colorimetrically by using the color development with ninhydrin. The results are shown in Table 20.

Aufarbeitung

  1. stirringshaking culture
  2. waitwas carried out at 30° C. for 72 hours

Quellen und Nachweise

ORD-Eintrag
ord-b513f79847d247d484ea46dfd5b6252f
Quelldatensatz
uspto-grants-1996_03
Patentnummer
US05498532

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