Potassium Hydroxide → Morphine

HRID 1458505

Reaktionsgleichung

Reaktionsgleichung von HRID 1458505
Cl.[CH:2]1[C:18]2[CH2:17][C@H:16]3[N:19]([CH2:21][CH2:22][C@@:8]45[C@H:15]3[CH:14]=[CH:13][C@H:11]([OH:12])[C@@H:9]4[O:10][C:6]([C:7]=25)=[C:4]([OH:5])[CH:3]=1)[CH3:20].[OH-].[K+].C1C(CN2C(=O)C=CC2=O)CCC(C(ON2C(=O)CCC2=O)=O)C1>CN(C)C=O>[CH:2]1[C:18]2[CH2:17][C@H:16]3[N:19]([CH2:21][CH2:22][C@@:8]45[C@H:15]3[CH:14]=[CH:13][C@H:11]([OH:12])[C@@H:9]4[O:10][C:6]([C:7]=25)=[C:4]([OH:5])[CH:3]=1)[CH3:20]

Komponenten

5

Edukt 3

HCID 14797Potassium HydroxideHCID 125175Succinimidyl 4-(N-maleimidomethyl)cyclohexanecarboxylateHCID 5464110Morphine Hydrochloride

Produkt 1

HCID 5288826Morphine

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 6228DimethylformamideLösungsmittel

Versuchsvorschrift

Morphine-HCTL (0.068 g, 1.3×10-4 mol, example 9) was dissolved in 5.7 ml of 70% dimethylformamide/30% water and 1.43 ml 1N potassium hydroxide was added. After 5 min the thiol concentration was determined by reaction with DTNB to be 16.9 mM. The morphine thiol -- (6.4 ml, 1.1×10-4 mol) was added with stirring to 26.7 ml SMCC-BSA, 15/1, (0.16 g, 2.4×10-6 mol). The container was purged with argon and the solution was stirred at room temperature for 2 hr. The protein solution was then dialyzed using a PYROSART module against 10 mM (2-(N-morpholino) ethane sulfonic acid, pH 5.77, and 29.5 ml of 5.39 mg/ml BSA-morphine was collected. The protein solution was frozen at -70° C.

Aufarbeitung

  1. concentrationAfter 5 min the thiol concentration
  2. customwas determined by reaction with DTNB
  3. customThe container was purged with argon
  4. customThe protein solution was then dialyzed
  5. customwas collected
  6. temperatureThe protein solution was frozen at -70° C.

Quellen und Nachweise

ORD-Eintrag
ord-a35981d8995646fbb08b45f0161ebb34
Quelldatensatz
uspto-grants-1998_12
Patentnummer
US05851776

Verknüpfte Notizen

Wird geladen…