Phenazine Methosulfate → 2-keto-L-Gulonic acid

HRID 1955507

Reaktionsgleichung

Reaktionsgleichung von HRID 1955507
[CH2:1]([OH:12])[C@H:2]([OH:11])[C@@H:3]([OH:10])[C@H:4]([OH:9])[C:5]([CH:7]=[O:8])=[O:6].C[N+]1C2C(=CC=CC=2)N=C2C=1C=CC=C2.C[O:29]S([O-])(=O)=O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH2:1]([OH:12])[C@H:2]([OH:11])[C@@H:3]([OH:10])[C@H:4]([OH:9])[C:5]([C:7]([OH:29])=[O:8])=[O:6]

Komponenten

4

Edukt 2

HCID 9285Phenazine MethosulfateHCID 10921067Sorbosone, L-

Produkt 1

HCID 1024242-keto-L-Gulonic acid

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 62657Potassium Phosphate, TribasicLösungsmittel

Versuchsvorschrift

A reaction mixture containing the purified aldehyde dehydrogenase (0.04 mg protein, and prepared according to Example 1), L-sorbosone (4 mg) and phenazine methosulfate (0.2 mg) in 1.0 ml of 0.5M potassium phosphate buffer (pH 7.0) was incubated for 1.0 hour at 25° C. with gentle shaking. As a result, 2-keto-L-gulonic acid was formed at the rate of about 2.3 mg/hour.

Aufarbeitung

  1. additionA reaction mixture containing

Quellen und Nachweise

ORD-Eintrag
ord-edbfe821f9b14615818ba2f9c293af75
Quelldatensatz
uspto-grants-1998_07
Patentnummer
US05776742

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