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Carbon Dioxide → Prostaglandin E2

HRID 2069545
Notizen
Druckvorschau

Reaktionsgleichung

Reaktionsgleichung von HRID 2069545
C1C=C2C(C3C=CC(O)=CC=3)(C3C=CC(O)=CC=3)OS(=O)(=O)C2=CC=1.N[C@H:27]([C:33]([OH:35])=[O:34])[CH2:28][CH2:29][C:30](=O)N.C(=O)=O.C[C@H:40]1[C@@H:61]([CH2:62]C2OC3C=CC(NC)=C(C(O)=O)C=3N=2)[O:60][C@:43]2([O:48][C@H:47]([C@@H:49]([C:51]([C:53]3NC=[CH:55][CH:54]=3)=[O:52])C)[C@H:46](C)[CH2:45][C@H:44]2C)[CH2:42][CH2:41]1>CS(C)=O>[CH3:62][CH2:61][CH2:40][CH2:41][CH2:42][C@H:43]([OH:60])/[CH:44]=[CH:45]/[C@@H:46]1[C@@H:53]([CH2:54]/[CH:55]=[CH:30]\[CH2:29][CH2:28][CH2:27][C:33]([OH:35])=[O:34])[C:51](=[O:52])[CH2:49][C@H:47]1[OH:48]
KomponentenVersuchsvorschriftAufarbeitungQuellen und NachweiseNotizen zur Reaktion

Komponenten

6

Edukt 4

HCID 280Carbon DioxideHCID 4766Phenol RedHCID 5961L-GlutamineHCID 11957499Calcimycin

Produkt 1

HCID 5280360Prostaglandin E2

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 679Dimethyl Sulfoxide
Lösungsmittel

Versuchsvorschrift

3T3 Swiss Albino fibroblast cell suspension (Phenol-red free DMEM with 10% FBS and 2 mM L-glutamine) was plated out into 96-well tissue culture plates (1×105 cells/mL, 100 μL/well). The cells were cultured overnight at 37° C., 5% CO2. The extracts and fractions were solubilised in DMSO, and diluted appropriately with media so the extracts were tested at a final concentration of 1000, 10, and 1 μg/mL; 5 μL of sample was added to each well. Cells+samples were incubated (37° C., 3 hours), before the addition of calcium ionophore A23187. Following 20 min incubation, the plate was centrifuged (1000 g, 3 min) and the supernatants were removed. Included on the plate was a positive control (aspirin; 100 μM), and DMSO control, both with and without calcium ionophore A23187.

Aufarbeitung

  1. additiondiluted appropriately with media so the extracts
  2. addition5 μL of sample was added to each well
  3. custom(37° C., 3 hours)
  4. waitFollowing 20 min
  5. custom(1000 g, 3 min)
  6. customthe supernatants were removed

Quellen und Nachweise

ORD-Eintrag
ord-488bcfd35b504fb5aa8dff68f4f9c95f
Quelldatensatz
uspto-grants-2012_10
DOI
10.6084/m9.figshare.5104873.v1
Patentnummer
US08299119B2

Notizen zur Reaktion

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