Reaktionsgleichung
C1C=C2C(C3C=CC(O)=CC=3)(C3C=CC(O)=CC=3)OS(=O)(=O)C2=CC=1.N[C@H:27]([C:33]([OH:35])=[O:34])[CH2:28][CH2:29][C:30](=O)N.C(=O)=O.C[C@H:40]1[C@@H:61]([CH2:62]C2OC3C=CC(NC)=C(C(O)=O)C=3N=2)[O:60][C@:43]2([O:48][C@H:47]([C@@H:49]([C:51]([C:53]3NC=[CH:55][CH:54]=3)=[O:52])C)[C@H:46](C)[CH2:45][C@H:44]2C)[CH2:42][CH2:41]1>CS(C)=O>[CH3:62][CH2:61][CH2:40][CH2:41][CH2:42][C@H:43]([OH:60])/[CH:44]=[CH:45]/[C@@H:46]1[C@@H:53]([CH2:54]/[CH:55]=[CH:30]\[CH2:29][CH2:28][CH2:27][C:33]([OH:35])=[O:34])[C:51](=[O:52])[CH2:49][C@H:47]1[OH:48]Komponenten
Edukt 4
Produkt 1
Reagenzien, Katalysatoren, Lösungsmittel 1
Versuchsvorschrift
3T3 Swiss Albino fibroblast cell suspension (Phenol-red free DMEM with 10% FBS and 2 mM L-glutamine) was plated out into 96-well tissue culture plates (1×105 cells/mL, 100 μL/well). The cells were cultured overnight at 37° C., 5% CO2. The extracts and fractions were solubilised in DMSO, and diluted appropriately with media so the extracts were tested at a final concentration of 1000, 10, and 1 μg/mL; 5 μL of sample was added to each well. Cells+samples were incubated (37° C., 3 hours), before the addition of calcium ionophore A23187. Following 20 min incubation, the plate was centrifuged (1000 g, 3 min) and the supernatants were removed. Included on the plate was a positive control (aspirin; 100 μM), and DMSO control, both with and without calcium ionophore A23187.
Aufarbeitung
- additiondiluted appropriately with media so the extracts
- addition5 μL of sample was added to each well
- custom(37° C., 3 hours)
- waitFollowing 20 min
- custom(1000 g, 3 min)
- customthe supernatants were removed
Quellen und Nachweise
- ORD-Eintrag
- ord-488bcfd35b504fb5aa8dff68f4f9c95f
- Quelldatensatz
- uspto-grants-2012_10
- Patentnummer
- US08299119B2
Verknüpfte Notizen
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