Hydrochloric Acid → Trehalose

HRID 2088327

Reaktionsgleichung

Reaktionsgleichung von HRID 2088327
[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[O:14][C@H:13]([CH2:15][OH:16])[C@@H:12]([OH:17])[C@H:11]([OH:18])[C@H:10]2[OH:19])[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22].O.O.Cl>O>[CH2:15]([OH:16])[C@H:13]1[O:14][C@H:9]([O:8][C@H:6]2[O:7][C@H:2]([CH2:1][OH:23])[C@@H:3]([OH:22])[C@H:4]([OH:21])[C@H:5]2[OH:20])[C@H:10]([OH:19])[C@@H:11]([OH:18])[C@@H:12]1[OH:17]

Komponenten

5

Edukt 3

HCID 313Hydrochloric AcidHCID 181978Trehalose, dihydrateHCID 178435178HCID 178435178, Verbindung

Produkt 1

HCID 7427Trehalose

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 962WaterLösungsmittel2×

Versuchsvorschrift

25.48 g of trehalose dihydrate was carefully weighed and transferred into a sterile polypropylene bottle, to which 350 ml of purified water was added at room temperature and stirred slowly until a clear solution was obtained. To the clear solution, 0.1 N HCl was added drop by drop to adjust the pH to 3.9, then the volume was adjusted with purified water to obtain 400 ml final volume. The pH was measured and found to be 4.2. The solution was filtered through a 0.22-micron filter and was used directly to dilute the protein solution.

Aufarbeitung

  1. additionwas added at room temperature
  2. customwas obtained
  3. customto obtain 400 ml final volume
  4. filtrationThe solution was filtered through a 0.22-micron
  5. filtrationfilter
  6. additionto dilute the protein solution

Quellen und Nachweise

ORD-Eintrag
ord-cd1d6bb629e64a15a977719921a72373
Quelldatensatz
uspto-grants-2013_05
Patentnummer
US08435943B2

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