Sucrose → 2-(1H-indol-2-yl)butanoic acid

HRID 2193992

Reaktionsgleichung

Reaktionsgleichung von HRID 2193992
C(O)[C@H]1O[C@H]([O:8][C@:9]2(CO)[O:13][C@H:12](CO)[C@@H:11](O)[C@@H:10]2O)[C@H](O)[C@@H](O)[C@@H]1O.[CH3:24][C:25](OCC1CS[C@@H]2[C@H](NC(/C(/C3N=C(N)SC=3)=N\OC)=O)C(=O)N2C=1C([O-])=O)=O.[Na+].[CH2:55]1[C@H:60](N)[C@@H:59](O[C@H]2O[C@H](CN)[C@@H](O)[C@H](O)[C@H]2O)[C@H:58](O)[C@@H:57](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](N)[C@H]2O)[C@@H:56]1[NH2:87].C(NC1N=C2C(NC=N2)=CN=1)C1C=CC=CC=1.O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>>[NH:87]1[C:56]2[C:57](=[CH:58][CH:59]=[CH:60][CH:55]=2)[CH:25]=[C:24]1[CH:10]([CH2:11][CH3:12])[C:9]([OH:8])=[O:13]

Komponenten

6

Edukt 5

HCID 5988Sucrose2×HCID 6032KanamycinHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 10695961Cefotaxime Sodium2×HCID 12922494N-Benzyl-7H-purin-2-amine

Produkt 1

HCID 190852562-(1H-indol-2-yl)butanoic acid

Versuchsvorschrift

To produce transgenic oilseed rape plants binary vectors in Agrobacterium tumefaciens C58C1-pGV2260 or Escherichia coli were used (Deblaere et al, 1984, Nucl. Acids. Res. 13, 4777-4788). For transforming oilseed rape plants (var. Drakkar, NPZ Nordeutsche Pflanzenzucht, Hohenlieth. Germany) a 1:50 dilution of an overnight culture of a positively transformed agrobacteria colony in Murashige-Skoog medium (Murashige and Skoog 1962 Physiol. Plant. 15, 473) containing 3% of saccharose (3MS medium) was used. Petioles or hypocotyledons of freshly germinated sterile rape plants (approx. 1 cm2 each) were incubated in a Petri dish with a 1:50 agrobacteria dilution for 5-10 minutes. This was followed by 3-day concubation in darkness at 25_C on 3MS medium containing 0.8% of Bacto-Agar. After three days, culturing was continued with 16 hours of light/8 hours of darkness and in a weekly cycle on MS medium containing 500 mg/l of Claforan (sodium cefotaxime), 50 mg/l of kanamycin, 20 microM of benzylaminopurine (BAP) and 1.6 g/l of glucose. Growing shoots were transferred onto MS medium containing 2% of saccharose, 250 mg/l of Claforan and 0.8% of Bacto-Agar. If after three weeks no roots had formed 2-indolylbutyric acid was added to the medium as a growth hormone for rooting purposes.

Aufarbeitung

  1. customTo produce transgenic oilseed rape plants binary vectors in Agrobacterium tumefaciens
  2. customa 1:50 dilution of an overnight culture
  3. waitThis was followed by 3-day
  4. additionconcubation in darkness at 25_C on 3MS medium containing 0.8% of Bacto-Agar
  5. waitAfter three days
  6. waitculturing was continued with 16 hours of light/8 hours of darkness and in a weekly cycle on MS medium

Quellen und Nachweise

ORD-Eintrag
ord-98727ccee4d14ac2b9fdecb070cd40bd
Quelldatensatz
uspto-grants-2012_12
Patentnummer
US08329993B2

Verknüpfte Notizen

Wird geladen…