Ethanol → Dopamine

HRID 247013

Reaktionsgleichung

Reaktionsgleichung von HRID 247013
O=C(O)CN([CH2:8][CH2:9][OH:10])CCO.N[CH2:13][C:14]([OH:16])=O.[CH3:17][C:18]#[N:19].O.[CH2:21](O)[CH3:22]>[Fe-3](C#N)(C#N)(C#N)(C#N)(C#N)C#N.[K+].[K+].[K+]>[NH2:19][CH2:18][CH2:17][C:21]1[CH:22]=[CH:8][C:9]([OH:10])=[C:14]([OH:16])[CH:13]=1

Komponenten

7

Edukt 5

HCID 702EthanolHCID 750GlycineHCID 962WaterHCID 6342AcetonitrileHCID 8761Bicine

Produkt 1

HCID 681Dopamine

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 178511488HCID 178511488, VerbindungKatalysator

Versuchsvorschrift

To the sample solution (1.0 ml) were added 2.0 ml of 0.05M Bicine buffer solution (pH 7.0) containing 0.5M glycine, 0.1 ml of 0.1M DPE solution (ethanol solvent) and 0.1 ml of 0.08% (W/V) potassium ferricyanide solution in sequence. After allowing to stand for more than 30 minutes at 37° C., 100 μl of the reaction liquid were injected into HPLC apparatus. Conditions of HPLC were as follows: column; Ultrasphere-ODS (4.6φ×150 mm, Beckman), eluate; CH3CN--H2O (47.5:52.5 W/V), flow rate 1 ml/min. For the fluorescence detection, Shimazu FLD-I (excitation 300-400 nm, fluorescence 450-800 nm) was employed. Under these conditions, reversed-phase partition type column as mentioned above, norepinephrine, epinephrine and isoproterenol gave a single peak respectively. Although dopamine gave two peaks of an intensity ratio of 9:1, the determination was possible with either of them. The elution was completed within 12 minutes, and the lower limit of detection was 10 pg at S/N ratio of 2.

Aufarbeitung

  1. waitto stand for more than 30 minutes at 37° C.
  2. custom100 μl of the reaction liquid
  3. customUnder these conditions, reversed-phase partition type column
  4. customgave a single peak respectively

Quellen und Nachweise

ORD-Eintrag
ord-3a7df77a6d0442cda7f637b74fb6ea55
Quelldatensatz
uspto-grants-1987_11
Patentnummer
US04705757

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