Reaktionsgleichung
C1C2(N(C3C=CC=CC=3)CNC2=O)CCN(CCC[C:21]([C:23]2[CH:28]=[CH:27][C:26](F)=CC=2)=[O:22])C1.C(O)[C:31](N)([CH2:34][OH:35])[CH2:32]O.Cl.[Na+].[Cl-].[Mg+2].[Cl-].[Cl-].CC([C@]1(O)C[C@H]2[N:51](C[C@H]3C4C2=CC=CC=4CCC2C=CC=CC3=2)CC1)(C)C>>[NH2:51][CH2:26][CH2:27][C:28]1[CH:32]=[CH:31][C:34]([OH:35])=[C:21]([OH:22])[CH:23]=1Komponenten
6
Versuchsvorschrift
0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H]spiperone in a final volume of 1 mL containing 50 mm Tris HCl, 120 mM NaCl and 1 mM MgCl2 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol. After 15 minutes of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL).
Aufarbeitung
- custom0.5 mL aliquots of the membrane preparation
- filtrationsamples were filtered rapidly through Whatman GF/C glass filters under negative pressure
- washwashed three times with ice-cold binding buffer (5 mL)
Quellen und Nachweise
- ORD-Eintrag
- ord-0c4bb657fe26408aac676cf4e32390ba
- Quelldatensatz
- uspto-grants-1993_11
- Patentnummer
- US05266572
Verknüpfte Notizen
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