Staurosporine → Staurosporine

HRID 438802

Reaktionsgleichung

Reaktionsgleichung von HRID 438802
CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4.Cc1noc(C)c1C=O>CC(=O)O.CC(=O)O[BH-](OC(C)=O)OC(C)=O.CC(C)[O-].CC(C)[O-].CC(C)[O-].CC(C)[O-].CN1CCCC1=O.[Na+].[Ti+4]>CO[C@@H]1[C@H](N(C)Cc2c(C)noc2C)C[C@H]2O[C@]1(C)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4

Komponenten

9

Edukt 2

HCID 44259StaurosporineHCID 2895763,5-Dimethyl-4-Isoxazolecarbaldehyde

Produkt 3

HCID 44259StaurosporineHCID 2895763,5-Dimethyl-4-IsoxazolecarbaldehydeHCID 178496690HCID 178496690, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 4

HCID 176Acetic AcidReagenzHCID 11026Titanium isopropoxideReagenzHCID 5049666Sodium triacetoxyborohydrideReagenzHCID 13387N-methyl-2-pyrrolidoneLösungsmittel7×

Reaktionsbedingungen

Temperatur
22 °C

Aufarbeitung

  1. additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
  2. additionThe reactions were quenched with 1 uL saturated aqueous NaHCO3.
  3. additionThe reactions were quenched with 1 uL water.
  4. additionThe reactions were diluted to approximately 10 mM.
  5. aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
  6. additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.

Quellen und Nachweise

ORD-Eintrag
ord-61e908aa17fa4b27b97d0fffae25962a
Quelldatensatz
Cernak miniaturized reductive amination
Originallink
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