Methyl iodide → 2'-O-methyl-5-methyl-2thiouridine

HRID 443326

Reaktionsgleichung

[CH3:1][C:2]1[C:3](=[O:18])[NH:4][C:5](=[S:17])[N:6]([CH:16]=1)[C@@H:7]1[O:15][C@H:12]([CH2:13][OH:14])[C@@H:10]([OH:11])[C@H:8]1[OH:9].[CH2:19]([Sn](=O)CCCC)CCC.CI>CN(C=O)C.[I-].C([N+](CCCC)(CCCC)CCCC)CCC.C(Cl)Cl>[CH3:19][O:9][C@@H:8]1[C@H:10]([OH:11])[C@@H:12]([CH2:13][OH:14])[O:15][C@H:7]1[N:6]1[CH:16]=[C:2]([CH3:1])[C:3](=[O:18])[NH:4][C:5]1=[S:17]

Komponenten

Reaktionsbedingungen

Temperatur: 50 °C

Versuchsvorschrift

To a stirred solution of 5-methyl-2-thiouridine (0.500 g, 1.8 mmol) in DMF (10 ml) is added dibutyltin oxide (0.500 g, 2.0 mmol), tetrabutyl ammonium iodide (0.738 g, 2 mmol), and methyl iodide (1.022 g, 7.2 mmol). The reaction flask is sealed and heated at 50° C. for 16 hours. The mixture is cooled and another portion of methyl iodide is added (1.022 g, 7.2 mmol) and the reaction heated for an additional 16 hours. At the end of this time, the reaction mixture is cooled to room temperature and diluted with methylene chloride and chromatographed using a methylene chloride/methanol gradient. The appropriate fractions are collected and concentrated to give 2'-O-methyl-5-methyl-2thiouridine.

Aufarbeitung

customThe reaction flask is sealed
temperatureThe mixture is cooled
temperaturethe reaction heated for an additional 16 hours
temperatureAt the end of this time, the reaction mixture is cooled to room temperature
customchromatographed
customThe appropriate fractions are collected
concentrationconcentrated

Quellen und Nachweise

ORD-Eintrag: ord-44de6741bd8440399f77569d184486d9
Quelldatensatz: uspto-grants-1999_04
DOI: 10.6084/m9.figshare.5104873.v1
Patentnummer: US05898031

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