Reaktionsgleichung
C(S)[C@@H](O)[C@H](O)CS.[C:9]([O-:14])(=[O:13])[C:10]([CH3:12])=[O:11].[O:15]=[CH:16][C@H:17]([C@H:19]([C@@H:21]([C@@H:23]([CH2:25][OH:26])[OH:24])[OH:22])[OH:20])[OH:18].OC(C1(O[C@@H]([C@@H]([C@@H](CO)O)O)[C@H](NC(C)=O)[C@@H](O)C1)O)=O>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[C:9]([OH:14])(=[O:13])[C:10]([CH2:12][C@H:25]([C@@H:23]([C@@H:21]([C@H:19]([C@H:17]([CH2:16][OH:15])[OH:18])[OH:20])[OH:22])[OH:24])[OH:26])=[O:11]Komponenten
6
Edukt 4
Produkt 1
Reagenzien, Katalysatoren, Lösungsmittel 1
Reaktionsbedingungen
- Temperatur
- 37 °C
Versuchsvorschrift
To a 10 mL potassium phosphate buffer (0.1 M, pH 7.5) were added 10 mM DTT, 0.5 M pyruvate, 0.1 M D-mannose and 1.5 mg NeuAc aldolase. The reaction mixture was shaken at 37° C. for three days. The reaction mixture was chromatographed with a Dowex-1 (HCO3−) resin, eluted with zero to 1 M ammonium bicarbonate gradient. The fractions containing KDN were pooled and lyophilized three times repeatedly to remove the volatile salt to give a 78 percent yield of KDN. The physical data of the product were are consistent with the reported.
Aufarbeitung
- customThe reaction mixture was chromatographed with a Dowex-1 (HCO3−) resin
- washeluted with zero to 1 M ammonium bicarbonate gradient
- additionThe fractions containing KDN
- customto remove the volatile salt
- customto give a 78 percent yield of KDN
Quellen und Nachweise
- ORD-Eintrag
- ord-8c7478989ffc47cbb8dbdb7a7938d4f1
- Quelldatensatz
- uspto-grants-2001_01
- Patentnummer
- US06168934A
Verknüpfte Notizen
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