L-Glutamine → 2',3'-Didehydro-2',3'-dideoxycytidine
Reaktionsgleichung
[CH3:1][C:2]1(C)S[C@@H]2[C@H](NC(CC3C=CC=CC=3)=O)[C:8](=[O:9])[N:4]2[C@H:3]1C([O-])=O.[K+].C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H]([NH:40]C(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O.N[C@H:66]([C:72]([OH:74])=O)[CH2:67][CH2:68][C:69](=[O:71])[NH2:70]>>[C@@H:69]1([N:70]2[CH:1]=[CH:2][C:3]([NH2:40])=[N:4][C:8]2=[O:9])[O:71][C@H:66]([CH2:72][OH:74])[CH:67]=[CH:68]1Komponenten
4
Versuchsvorschrift
Nucleoside Analogs: One millimolar solutions were prepared in glass distilled water and filter sterilized through 0.2μ filters. Subsequent dilutions were prepared in RPMI 1640 medium containing 15% fetal calf serum, penicillin, streptomycin and glutamine.