Citric Acid → Thromboxane

HRID 615598

Reaktionsgleichung

Reaktionsgleichung von HRID 615598
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][C@H:6](O)/[CH:7]=[CH:8]/[C@@H:9]1[C@@H:13]([CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH2:18][CH2:19][C:20](O)=O)[C@H:12]2O[O:24][C@@H:10]1[CH2:11]2.C(O)(=O)CC(CC(O)=O)(C(O)=O)O>CCOCC>[CH3:20][CH2:19][CH2:18][CH2:17][CH2:16][CH2:15][CH2:14][C@@H:13]1[C@@H:9]([CH2:8][CH2:7][CH2:6][CH2:5][CH2:4][CH2:3][CH2:2][CH3:1])[O:24][CH2:10][CH2:11][CH2:12]1

Komponenten

4

Edukt 2

HCID 311Citric AcidHCID 445049Prostaglandin H2

Produkt 1

HCID 114873Thromboxane

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 3283Diethyl EtherLösungsmittel

Versuchsvorschrift

To a 12×75 mm tube containing [1-14C] PGH2 (2 μg, 0.01 μCi or 0.05 μCi) and inhibitor (1 mM final assay concentration, in 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung mocrosomes (25 μl, containing 400 μg protein) or an aliquot of human platelet microsomes (25 μl, containing ≅130 g protein). The solution was incubated in a 22° water bath for 2 min.; ether (1 ml) and citric acid (25 μMoles) were added, and the tube plunged into an acetone-dry ice bath. The ether extract was transferred to a 1.0 ml Reacti-vial, and the volume reduced under nitrogen to ≅50 μl.

Aufarbeitung

  1. concentrationassay concentration
  2. additionin 10 μl of ethanol) in 225 μl of phosphate buffer (0.1M, pH 7.5), was added to an aliquot of guinea pig lung mocrosomes (25 μl, containing 400 μg protein)
  3. additionan aliquot of human platelet microsomes (25 μl, containing ≅130 g protein)
  4. customwas incubated in a 22°
  5. customfor 2 min.
  6. customthe tube plunged into an acetone-dry ice bath
  7. extractionThe ether extract

Quellen und Nachweise

ORD-Eintrag
ord-f987717a68094bb3ac24ef7c396c803e
Quelldatensatz
uspto-grants-1985_02
Patentnummer
US04500540

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