[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[ClH:9]>O>[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[ClH:9]6.057 g of Tris base(molecular weight 121.14 g) was accurately weighed into a 1 litre volumetric flask. 800 ml of deionised water was added, it's pH adjusted to pH8 with concentrated HCl and then diluted to volume with de-ionised water. Buffer was stored at 4° C.
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