Urea → 2-keto-L-Gulonic acid

Reaction equation

[OH:1][CH2:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].NC(N)=[O:15].OP([O-])(O)=O.[K+].C([O-])([O-])=O.[Ca+2]>>[CH2:2]([OH:1])[C@H:3]([OH:4])[C@@H:5]([OH:6])[C@H:7]([OH:8])[C:9]([C:11]([OH:15])=[O:12])=[O:10]

Participants

Reactant 4

HCID 1176Urea

HCID 5780Sorbitol

HCID 10112Calcium Carbonate

HCID 516951Monobasic potassium phosphate

Product 1

HCID 1024242-keto-L-Gulonic acid

Procedure

Five ml of the resulting individual seed cultures as prepared above was inoculated into a 500 ml of Erlenmeyer flask containing 50 ml of production medium (PM): D-sorbitol 8%, corn steep liquor 1%, urea 1.5% (sterilized separately), KH2PO4 0.1%, MgSO4.7H2O 0.01%, CaCO3 0.6% and antifoam 0.1% (pH 7.0 before sterilization) and incubated at 30° C. for 4 days with shaking (180 rpm). Table 1 summarizes the results of the quantitative determination of 2-keto-L-gulonic acid of the cultured broths as assayed by high performance liquid chromatography.