Urea → 2-keto-L-Gulonic acid

HRID 1033722

Reaction equation

[OH:1][CH2:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].NC(N)=[O:15].OP([O-])(O)=O.[K+].C([O-])([O-])=O.[Ca+2]>>[CH2:2]([OH:1])[C@H:3]([OH:4])[C@@H:5]([OH:6])[C@H:7]([OH:8])[C:9]([C:11]([OH:15])=[O:12])=[O:10]

Participants

Reactant 4

HCID 1176Urea

HCID 5780Sorbitol

HCID 10112Calcium Carbonate

HCID 516951Monobasic potassium phosphate

Product 1

HCID 1024242-keto-L-Gulonic acid

Procedure

Five ml of the resulting individual seed cultures as prepared above was inoculated into a 500 ml of Erlenmeyer flask containing 50 ml of production medium (PM): D-sorbitol 8%, corn steep liquor 1%, urea 1.5% (sterilized separately), KH2PO4 0.1%, MgSO4.7H2O 0.01%, CaCO3 0.6% and antifoam 0.1% (pH 7.0 before sterilization) and incubated at 30° C. for 4 days with shaking (180 rpm). Table 1 summarizes the results of the quantitative determination of 2-keto-L-gulonic acid of the cultured broths as assayed by high performance liquid chromatography.

Workup

customFive ml of the resulting individual seed cultures as prepared
additioncontaining 50 ml of production medium (PM)

Sources & evidence

ORD record: ord-5749d793ee5347d998bda00a14d9639e
Source dataset: uspto-grants-1994_05
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05312741

Linked notes

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