o-Dianisidine dihydrochloride → Hexose

HRID 1255381

Reaction equation

HRID 1255381 reaction equation
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].COC1C=C(C2C=CC(N)=C(OC)C=2)C=CC=1N.Cl.Cl>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[CH2:11]([OH:12])[CH:9]1[O:10][CH:2]([OH:1])[CH:3]([OH:4])[CH:5]([OH:6])[CH:7]1[OH:8]

Participants

4

Reactant 2

HCID 62311o-Dianisidine dihydrochlorideHCID 64689Beta-D-Glucose

Product 1

HCID 206Hexose

Reagents, catalysts & solvents 1

HCID 24243Trisodium PhosphateSolvent2×

Procedure

The assay was based on the method described by Sullivan and Ikawa (Biochimica et Biophysica Acta, 1973, 309:11-22), but modified to run in microtiter plates. An assay mixture contained 150 μl β-D-glucose (0.1 M in 0.1 M sodium phosphate buffer, pH 6.3), 120 μl 0.1 M sodium phosphate buffer, pH 6.3, 10 μl o-dianisidine-dihydrochloride (Sigma D-3252, 3.0 mg/ml in H2O), 10 μl peroxidase (POD) (Sigma P-8125, 0.1 ml in 0.1 M sodium phosphate buffer, pH 6.3) and 10 μl enzyme (HOX) solution. Blanks were made by adding buffer in place of enzyme solution.

Workup

  1. additionby adding buffer in place of enzyme solution

Sources & evidence

ORD record
ord-ac4e11fff7634ccc90f49a0aade7629e
Source dataset
uspto-grants-2013_06
Patent number
US08460723B2

Linked notes

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