Udp-Glucose → Sucrose
Reaction equation
C1N([CH2:7][CH2:8][OH:9])CCN(CCS(O)(=O)=O)C1.[OH-].[Na+].C1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP([O:40][C@H:41]3[O:46][C@H:45]([CH2:47][OH:48])[C@@H:44]([OH:49])[C@H:43]([OH:50])[C@H:42]3[OH:51])(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=1.CC1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP(OC3[O:83][C@H:82]([CH2:84][OH:85])[C@@H:81]([OH:86])[C@H:80]([OH:87])[C@H]3O)(O)=O)(O)=O)[C@@H](O)C2)C=1>>[CH2:47]([OH:48])[C@H:45]1[O:46][C@H:41]([O:40][C@:7]2([CH2:8][OH:9])[O:83][C@H:82]([CH2:84][OH:85])[C@@H:81]([OH:86])[C@@H:80]2[OH:87])[C@H:42]([OH:51])[C@@H:43]([OH:50])[C@@H:44]1[OH:49]Participants
4
Procedure
It was found that the hitherto used hepes-NaOH-buffer was most suitable for the synthesis of UDP-glucose and TDP-glucose. For both enzymes, the Mops-buffer and the TES-buffer gave 60 to 80% residual activity. The commercial enzyme indicated, by contrast to the rice enzyme, in TEA-buffer, an about 30 to 40% higher residual activity. In the remaining buffers, both enzymes had a residual activity of less than 50%.