Dianisidine → 2-Naphthol

HRID 1576739

Reaction equation

HRID 1576739 reaction equation
COC1C=[C:7]([C:9]2[CH:14]=[CH:13][C:12](N)=[C:11]([O:16]C)[CH:10]=2)[CH:6]=[CH:5][C:4]=1N.[OH-].[Na+]>>[CH:10]1[C:9]2[C:14](=[CH:4][CH:5]=[CH:6][CH:7]=2)[CH:13]=[CH:12][C:11]=1[OH:16]

Participants

3

Reactant 2

HCID 8411DianisidineHCID 14798Sodium Hydroxide

Product 1

HCID 86632-Naphthol

Procedure

Silica particles were derivatized with o-dianisidine by Method No. 2. After drying, 1.0 gm of the carrier was diazotized with sodium nitrite. After diazotization the derivative was washed several times with borate (0.03M) buffered saline (0.15M) pH 8.0. The derivative was then reacted with 1.5 ml of T4 antisera (titer 1:10000) at 4°C. with stirring. The pH was maintained between 8.0 - 8.5 with 0.1N NaOH. After 30 minutes the pH stabilized at 8.2 and the reaction was stirred slowly at 4°C. overnight. After this time the composite gave a negative β-napthol test indicating no more remaining functional diazo groups. The preparation was then washed (4X) with phosphate-buffer (0.03M) saline (0.15M) containing 0.1% bovine serum albumin (PBS-BSA).

Workup

  1. customAfter drying
  2. washAfter diazotization the derivative was washed several times with borate (0.03M)
  3. customThe derivative was then reacted with 1.5 ml of T4 antisera (titer 1:10000) at 4°C.
  4. stirringthe reaction was stirred slowly at 4°C. overnight

Sources & evidence

ORD record
ord-2178f3db996a407b8d5833f978e270e8
Source dataset
uspto-grants-1976_09
Patent number
US03983000

Linked notes

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