Water → FA amoxicillin

HRID 1629636

Reaction equation

HRID 1629636 reaction equation
[CH3:1][C:2]1([CH3:25])[S:6][C@@H:5]2[C@H:7]([NH:10][C:11]([C@H:13]([NH2:21])[C:14]3[CH:15]=[CH:16][C:17]([OH:20])=[CH:18][CH:19]=3)=[O:12])[C:8](=[O:9])[N:4]2[C@H:3]1[C:22]([OH:24])=[O:23].O.C(N=C=NCCCN(C)C)C.[CH:38]1[C:43]([NH2:44])=[CH:42][C:41]2[C:45]([O:47][C:48]3([C:58]4[CH:59]=[CH:60][C:61]([OH:63])=[CH:62][C:57]=4[O:56][C:50]4[CH:51]=[C:52]([OH:55])[CH:53]=[CH:54][C:49]3=4)[C:40]=2[CH:39]=1)=[O:46]>C(#N)C>[CH:38]1[C:43]([NH2:44])=[CH:42][C:41]2[C:45]([O:47][C:48]3([C:58]4[CH:59]=[CH:60][C:61]([OH:63])=[CH:62][C:57]=4[O:56][C:50]4[CH:51]=[C:52]([OH:55])[CH:53]=[CH:54][C:49]3=4)[C:40]=2[CH:39]=1)=[O:46].[CH3:1][C:2]1([CH3:25])[S:6][C@@H:5]2[C@H:7]([NH:10][C:11]([C@H:13]([NH2:21])[C:14]3[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][CH:15]=3)=[O:12])[C:8](=[O:9])[N:4]2[C@H:3]1[C:22]([OH:24])=[O:23]

Participants

6

Reactant 4

HCID 962WaterHCID 159081-Ethyl-3-(3'-dimethylaminopropyl)carbodiimideHCID 33613Amoxicillin2×HCID 768455-Aminofluorescein4×

Product 1

HCID 131731698FA amoxicillin

Reagents, catalysts & solvents 1

HCID 6342AcetonitrileSolvent

Procedure

Fluoresceinamine (FA; 0.0583 g) was dissolved in 1.0 ml acetonitrile to from a FA solution. Amoxicillin (0.1 g) was dissolved in 30 ml deionized water, and mixed with the aforesaid FA solution. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (0.0408 g) was added thereto and magnetically stirred at room temperature in the dark for 12 hours to make FA to graft to the carboxyl group of amoxicillin to form a fluorescent-labeled FA-amoxicillin. And, finally, the sample was freeze-dried to collect the fluorescent-labeled FA-amoxicillin.

Sources & evidence

ORD record
ord-adb1b15315a040e0ac32635795454c18
Source dataset
uspto-grants-2014_07
Patent number
US08790695B2

Linked notes

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