Thymidine → Aflatoxin M1

HRID 1762967

Reaction equation

HRID 1762967 reaction equation
C1(N=C=N[CH:10]2[CH2:15][CH2:14][CH2:13][CH2:12][CH2:11]2)CCCCC1.OO.[C:18]([CH2:23][C:24]([O-:26])=O)([C:20]([OH:22])=O)=[O:19].N1[C:35](=[O:36])[C:34]2NC=N[C:30]=2N=C1.NNC1N[C:47](=[O:49])C2C(=NC=CN=2)N=1.[C@@H]1(N2[CH:66]=[C:64](C)C(=O)NC2=O)O[C@H](CO)[C@@H](O)C1.CC(CCCC(C)CCCC(C)CCCC(C)C)C.CC1(C)S[C@@H]2[C@H](NC(CC3C=CC=CC=3)=O)C(=O)N2[C@H]1[C:105]([O-:107])=[O:106].[K+].C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H](O[C@@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2NC)[C@@]1(O)C=O.C([O-])(=O)C(C)=O.[Na+].CCC1OC(C2C=CC(Cl)=CC=2Cl)(CN2N=CN=C2)OC1>O1CCCC1.CN(C)C=O>[CH3:47][O:49][C:10]1[CH:11]=[C:12]2[O:22][C@H:20]3[O:26][CH:24]=[CH:23][C@@:18]3([OH:19])[C:13]2=[C:14]2[C:15]=1[C:30]1[CH2:66][CH2:64][C:35](=[O:36])[C:34]=1[C:105]([O:107]2)=[O:106]

Participants

12

Reactant 9

HCID 5789ThymidineHCID 15979PristaneHCID 91673EtaconazoleHCID 3260017Oxaloacetate IonHCID 20039623Hydrogen peroxide dicyclohexylcarbodiimideHCID 23662274Sodium pyruvateHCID 135398638HypoxanthineHCID 136025254Amino-pterinHCID 178431920HCID 178431920, compound

Product 1

HCID 15558498Aflatoxin M1

Reagents, catalysts & solvents 2

HCID 6228DimethylformamideSolventHCID 8028TetrahydrofuranSolvent

Procedure

Materials: All inorganic chemicals and organic solvents were of reagent grade or better. Bovine serum albumin (BSA) (fatty acid free and fraction V) ovalbumin (OA) (crude and fraction VII), polyethylene sorbitan monolaurate (Tween 20), 2,2'-azino-di-(3-ethylbentzthiazoline)sulfonic acid (ABTS), hydrogen peroxide dicyclohexylcarbodiimide, N-hydroxysuccidimide, dimethylformamide (DMF), polyethylene glycol (PEG) (MW 1450), insulin, oxaloacetate, hypoxanthine (H), amino-pterin (A), thymidine (T) and 2,6,10,14 tetramethylpentadecane (pristane), horseradish peroxidase (HRP) Type VI were purchased from Sigma Chemical Company (St. Louis, Mo.); tetrahydrofuran from Aldrich Chemical Company (Milwaukee, Wis.); Freund's complete and incomplete adjuvants from Difco (Detroit, Mich.); goat anti-mouse IgG conjugated to horseradish peroxidase from Cooper Biomedical (Malvern, Pa.); Dulbecco's Modified Eagle's Medium (DMEM), Penicillin/Streptomycin Solution (Pen/Strep) (100,000 U/ml), NCTC Medium 135, fetal bovine serum (FBS), and sodium pyruvate from Gibco Laboratories (Grand Island, N.Y.); microculture plates (96- and 24-well plates) from Costar (Cambridge, Mass.); microtiter plates from NUNC, Vangard International (Neptune, N.J.). Aflatoxins B1, B2, G1, G2 were purchased from Sigma Chemical Company (St. Louis, Mo.); aflatoxin M1 was generously provided by the Department of Botany and Plant Pathology, Michigan State University, East Lansing, Mich. Subclass identification kit was purchased from Boehringer Mannheim Biochemicals (Indianapolis, Ind.). The myeloma cell line P3/NS 1/1-Ag-4 (NS-1) (ATCC TIB 18) was purchased from The American Type Culture Collection (Rockville, Md.). Mice were purchased from Charles River Laboratories (Wilmington, Mass.).

Sources & evidence

ORD record
ord-d964f58be0eb4db0bb995c3de33c36a3
Source dataset
uspto-grants-1989_05
Patent number
US04835100

Linked notes

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