Dicyclohexylcarbodiimide → HCID 71243534, compound

To a solution of 4-(((1S,2S)-1-hydroxy-2,3-dihydro-1H,1′H-[2,2′-biinden]-2-yl)methyl)benzoic acid (200 mg, 0.52 mmol), DCC (129 mg, 0.62 mmol) and DMAP (6 mg, 0.052 mmol) in ethyl acetate (10 mL), was added N-[(9H-fluoren-9-ylmethoxy)carbonyl]-L-leucine (183 mg, 0.52 mmol) and then stirred at room temperature for 12 h. The solids were filtered, washed with ethyl acetate (25 ml) and the combined filtrate was washed with 1.5 N HCl (25 mL), water (25 mL), brine (10 mL), dried over anhydrous Na2SO4. The organic layer was evaporated under reduced pressure and the residue 1 (210 mg) was dissolved in dry THF (5 mL) and cooled to 0° C. Dimethylamine (5 mL, 20% in THF) was added drop wise at N2 atmosphere and the reaction mixture was slowly allowed to stir at room temperature for 1 h. The reaction mixture was evaporated under reduced pressure and the residue was purified by CombiFlash using 10% methanol in chloroform as an eluent to yield 120 mg (47%) of the title compound as an off white solid.

1. filtrationThe solids were filtered
2. washwashed with ethyl acetate (25 ml)
3. washthe combined filtrate was washed with 1.5 N HCl (25 mL), water (25 mL), brine (10 mL)
4. dry with materialdried over anhydrous Na2SO4
5. customThe organic layer was evaporated under reduced pressure
6. dissolutionthe residue 1 (210 mg) was dissolved in dry THF (5 mL)
7. temperaturecooled to 0° C
8. additionDimethylamine (5 mL, 20% in THF) was added drop wise at N2 atmosphere
9. stirringto stir at room temperature for 1 h
10. customThe reaction mixture was evaporated under reduced pressure
11. customthe residue was purified by CombiFlash

ORD record

ord-7a9fb75074cb4641b5fb6c45300345d1

Source dataset

uspto-grants-2016_02

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US09260376B2