C([O:3][C:4]([CH:6]1[CH2:11][CH2:10][CH2:9][CH:8]([N:12]2[C:21]3[C:16](=[CH:17][CH:18]=[N:19][CH:20]=3)[C:15]3=[N:22][O:23][C:24]([CH3:25])=[C:14]3[C:13]2=[O:26])[CH2:7]1)=[O:5])C.[OH-].[Na+].C(O)C.Cl>O.O1CCCC1>[CH3:25][C:24]1[O:23][N:22]=[C:15]2[C:16]3[C:21](=[CH:20][N:19]=[CH:18][CH:17]=3)[N:12]([CH:8]3[CH2:9][CH2:10][CH2:11][CH:6]([C:4]([OH:5])=[O:3])[CH2:7]3)[C:13](=[O:26])[C:14]=12Combine 3-(3-methyl-4-oxo-5H-2-oxa-1,5,7-triaza-cyclopenta[a]naphthalen-5-yl)-cyclohexane-carboxylic acid ethyl ester (620 mg, 1.75 mmol) with aqueous sodium hydroxide (2N, 5.0 mL, 10.0 mmol), ethanol (2 mL), and tetrahydrofuran (2 mL) and stir at ambient temperature until hydrolysis is complete. Add water and adjust to approx. pH 3.0 with aqueous hydrochloric acid. Extract with dichloromethane and dry over sodium sulfate and concentrate in vacuo to give the desired acid as a tan solid (500 mg, 88%).
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