L-Alanine → L-Phenylalanine
Reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11]O)O)O)O)O.[NH2:13][C@H:14]([C:16]([OH:18])=[O:17])[CH3:15]>>[NH2:13][C@@H:14]([C:16]([OH:18])=[O:17])[CH2:15][C:2]1[CH:11]=[CH:9][CH:7]=[CH:5][CH:3]=1.[NH2:13][C@H:14]([C:16]([OH:18])=[O:17])[CH2:15][C:2]1[CH:11]=[CH:9][CH:7]=[CH:5][CH:3]=1Participants
4
Procedure
The amount of glucose fed was 2021 g over 52 hours. The fermentation medium included a D-, L-alanine feed whereby a total of 1400 mls of 167 g/l D-, L-alanine was fed at a rate of 1.9 ml/min starting 12 hrs from the beginning of the fermentation. In addition, L-phenylalanine was fed at the same concentration and rate as the D-, L-alanine. The fermentation resulted in 13.66 g/l of D-phenylalanine and 0.87 g/l L-phenylalanine being produced.