L-(-)-Sorbose → Sorbosone, L-

HRID 2073158

Reaction equation

HRID 2073158 reaction equation
[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4].P([O-])([O-])([O-])=O.[K+].[K+].[K+].C[N+]1C2C(=CC=CC=2)N=C2C=1C=CC=C2.COS([O-])(=O)=O>O>[CH2:11]([OH:12])[C@H:9]([OH:10])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C:3]([CH:2]=[O:1])=[O:4]

Participants

5

Reactant 3

HCID 6904L-(-)-Sorbose2×HCID 9285Phenazine MethosulfateHCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 10921067Sorbosone, L-

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

The reaction mixture containing 100 ml of cell free extract of UV-10, FERM BP-1267 (total enzyme activity, 123 units), as prepared by the manner as described in steps (1) to (2) of Example 1, 50 ml of 0.5M potassium phosphate buffer (PH 6.0), 50 ml of 1M L-sorbose solution, 10 ml of 0.2M phenazine methosulfate solution and 290 ml of water was incubated at 30° C. with gentle shaking. As a result, L-sorbosone was formed with the rate of 142 mg/hr.

Workup

  1. additionThe reaction mixture containing 100 ml of cell
  2. extractionfree extract of UV-10, FERM BP-1267 (total enzyme activity, 123 units)
  3. customas prepared by the manner
  4. customwas incubated at 30° C. with gentle shaking

Sources & evidence

ORD record
ord-09d3d758083f48dcb63646c3023180fe
Source dataset
uspto-grants-1990_04
Patent number
US04916069

Linked notes

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