L-(-)-Sorbose → Sorbosone, L-

HRID 2073159

Reaction equation

HRID 2073159 reaction equation
[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4].P([O-])([O-])([O-])=O.[K+].[K+].[K+]>C[N+]1C2C(=CC=CC=2)N=C2C=1C=CC=C2.COS([O-])(=O)=O>[CH2:11]([OH:12])[C@H:9]([OH:10])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C:3]([CH:2]=[O:1])=[O:4]

Participants

4

Reactant 2

HCID 6904L-(-)-Sorbose3×HCID 62657Potassium Phosphate, Tribasic

Product 1

HCID 10921067Sorbosone, L-

Reagents, catalysts & solvents 1

HCID 9285Phenazine MethosulfateSolvent

Procedure

The reaction mixture containing 400 ml of purified membrane-bound L-sorbose dehydrogenase (total activity, 120 units), as prepared by the manner as described in steps (1) to (5) of Example 1, 50 ml of 0.5M potassium phosphate buffer (pH 6.0). 50 ml of 1M L-sorbose solution and 10 ml of 0.2M phenazine methosulfate solution was incubated at 30° C. with gentle shaking. As a result, L-sorbosone was formed with the rate of 224 mg/hr.

Workup

  1. customas prepared by the manner
  2. customwas incubated at 30° C. with gentle shaking

Sources & evidence

ORD record
ord-9f0bf67697f44edd8787cd0b3876adcd
Source dataset
uspto-grants-1990_04
Patent number
US04916069

Linked notes

Loading…