Cl[C:2]1[N:10]=[C:9](S(CCC)(=O)=O)[N:8]=[C:7]2[C:3]=1[N:4]=[CH:5][N:6]2[C:17]1[CH:22]=[CH:21][C:20]([Cl:23])=[CH:19][CH:18]=1.[NH:24]1[CH2:31][CH2:30][CH2:29][C@H:25]1[C:26]([NH2:28])=[O:27].[CH:32]([N:35](CC)C(C)C)(C)C>O1CCCC1>[Cl:23][C:20]1[CH:19]=[CH:18][C:17]([N:6]2[CH:5]=[N:4][C:3]3[C:7]2=[N:8][C:9]([C:32]#[N:35])=[N:10][C:2]=3[N:24]2[CH2:31][CH2:30][CH2:29][C@H:25]2[C:26]([NH2:28])=[O:27])=[CH:22][CH:21]=1A solution of the product from step (iii) (0.2 g), L-prolinamide (0.062 g) and N,N-diisopropylethylamine (0.19 ml) in tetrahydrofuran (10 ml) was stirred at room temperature for 24 h. The solvent was removed, the residue dissolved in N,N-dimethylformamide (10 ml) and sodium cyanide (0.05 g) added and heated at 90° C. for 10 h. The mixture was partitioned between ethyl acetate and water, the organics dried (MgSO4) and evaporated under reduced pressure. The residue was purified by RPHPLC. Yield 0.062 g
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