NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

HRID 223250

Reaction equation

C(O)C(N)(CO)CO.Cl.[CH:10]1[CH:15]=[N+:14]([C@@H:16]2[O:20][C@H:19]([CH2:21][O:22][P:23]([O:26][P:27]([O:30][CH2:31][C@H:32]3[O:36][C@@H:35]([N:37]4[C:41]5[N:42]=[CH:43][N:44]=[C:45]([NH2:46])[C:40]=5[N:39]=[CH:38]4)[C@H:34]([O:47][P:48]([OH:51])([OH:50])=[O:49])[C@@H:33]3[OH:52])([OH:29])=[O:28])([OH:25])=[O:24])[C@@H:18]([OH:53])[C@H:17]2[OH:54])[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH:11]=1>C(O)(C)C>[CH:43]1[N:44]=[C:45]([NH2:46])[C:40]2[N:39]=[CH:38][N:37]([C@@H:35]3[O:36][C@H:32]([CH2:31][O:30][P:27]([O:26][P:23]([O:22][CH2:21][C@H:19]4[O:20][C@@H:16]([N:14]5[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH2:11][CH:10]=[CH:15]5)[C@H:17]([OH:54])[C@@H:18]4[OH:53])([OH:25])=[O:24])([OH:29])=[O:28])[C@@H:33]([OH:52])[C@H:34]3[O:47][P:48]([OH:51])([OH:50])=[O:49])[C:41]=2[N:42]=1

Participants

Conditions

Temperature: 45 °C

Procedure

The gel was used to prepare a sepharose-alcohol dehydrogenase column (0.4×4.0 cm) which was then immersed in a water bath maintained at 45° C. A 0.01 M Tris-HCl solution (pH 9.0) containing 2.5 mg/ml NADP and 0.25 v/v isopropanol was applied to the column at a rate of 0.25 ml/min.; the treated solution was collected in a cooled ethanol precipitation vessel. Several milliliters of petroleum ether were added to 20 ml of the eluted solution and the precipitate was collected by centrifugation.

Workup

customwas then immersed in a water bath
customthe treated solution was collected in a cooled ethanol precipitation vessel
additionSeveral milliliters of petroleum ether were added to 20 ml of the eluted solution
customthe precipitate was collected by centrifugation

Sources & evidence

ORD record: ord-509c63b7c80e462a862f4e786f5ff757
Source dataset: uspto-grants-1982_10
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US04352885

Linked notes

Loading…