(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal → D-Fructose-1,6-diphosphate dicalcium salt
Reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[P:13]([O-:20])([O-:19])([O:15]C(=O)C)=O.[Cl-].[Ca+2:22].[Cl-]>CC(C)=O>[P:13]([O:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][O:12][P:13]([O-:20])([O-:19])=[O:15])[OH:10])[OH:8])[OH:6])=[O:4])([O-:20])([O-:19])=[O:15].[Ca+2:22].[Ca+2:22]Participants
Conditions
- Temperature
- 30 °C
Procedure
Propionibacterium freudenreichii IFO12425 was inoculated into 10 liters of a culture medium (pH: 6.5) containing 0.35% of glucose, 0.30% of peptone, 0.30% of yeast extract, and 0.05% of KH2PO4. This bacteria was cultured at 30° C. using a jar fermenter; 20 g of wet microorganisms were collected by centrifugation and immersed in acetone for 2 hours. Separately, 30 liters of tris-HCl solution prepared in the same manner as in Example 1 was placed in a 70-liter reaction vessel. To the tris-HCl solution was added 20 g of the acetone-treated microorganism body. The resulting solution was maintained at 30° C., and 20 liters of acetyl phosphate prepared as in the Reference Example was added in 5-liter portions. The reaction solution yielded 3.6 kg of dicalcium fructose-1,6-diphosphate salt following treatment with calcium chloride in the same manner as described in Example 1.
Workup
- extractionextract
- customwas cultured at 30° C.
- custom20 g of wet microorganisms were collected by centrifugation
- customSeparately, 30 liters of tris-HCl solution prepared in the same manner as in Example 1
- customwas placed in a 70-liter reaction vessel
- additiontreated microorganism body
- additionwas added in 5-liter portions
Sources & evidence
- ORD record
- ord-c5e0b335a09b4626bc79d6ffc1f90007
- Source dataset
- uspto-grants-1992_03
- Patent number
- US05094947
Reaction notes
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